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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

DOI :

10.3791/55185-v

11:37 min

June 22nd, 2017

June 22nd, 2017

15,639 Views

1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University

Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.

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QTL Mapping

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