Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
2:07
T Cell Isolation from the Splenocyte Cell Suspension
3:26
T-cell Activation and the Induction of GFP Expression
4:42
High Throughput Screening of Small Molecule
6:05
Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
7:56
Conclusion
Transcript
The overall goal of this florescence-based lymphocyte assay is to identify new small molecule immunomodulators. This method can help answer key questions in the field of immunology about the mechanism of lymphocyte activation and inhibition. The m
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Vi presenterar i den aktuella studien en ny fluorescensbaserad analys med användning av lymfocyter härledda från en transgen mus. Denna analys är lämplig för high-throughput screening (HTS) av små molekyler utrustade med egenskap av antingen inhibering eller främjande av lymfocytaktivering.