Large Scale Preparation of Endotoxin-free Plasmid DNA
1:36
Acufection Procedure and Post-operative Care
3:17
Imiquimod Treatment of IL-15ΔE7-acufected Skin
4:06
Mouse Skin Homogenation
5:20
Immunohistochemical Staining of the Skin Section
7:23
Results: Acufection Enables an Efficient Gene Trasfer While Inducing Minimal Skin Damage
8:37
Conclusion
Transcript
The overall goal of this procedure is to deliver immune related genes into skin tissue to delineate the functional role of a specific gene in a cutaneus inflammation. This method can aid in discovering the function of a novel gene in the skin. For
Sign in or start your free trial to access this content
This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.