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DOI :
10.3791/55206-v
April 19th, 2017
Chapters
0:05
Title
0:40
Large Scale Preparation of Endotoxin-free Plasmid DNA
1:36
Acufection Procedure and Post-operative Care
3:17
Imiquimod Treatment of IL-15ΔE7-acufected Skin
4:06
Mouse Skin Homogenation
5:20
Immunohistochemical Staining of the Skin Section
7:23
Results: Acufection Enables an Efficient Gene Trasfer While Inducing Minimal Skin Damage
8:37
Conclusion
이 프로토콜은 마우스의 피부에 벌거 벗은 플라스미드 DNA를 표현하기위한 비용 효율적인 대안이다. 프로토콜의 전반적인 목표는 피부 염증의 특정 유전자의 기능적 역할을 묘사하기 위해 피부 조직에 면역 관련 유전자를 제공하는 것입니다.
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