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JoVE Journal

Developmental Biology

This content is Open Access.

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

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10:39 min

April 22nd, 2017

April 22nd, 2017

11,184 Views

0:05

Title

0:50

Pre-selection of Females and Preparation of Maturation Medium

2:29

Dissection of Oocytes and Initiation of In Vitro Culture

4:56

Oocyte Microinjection

5:52

Oocyte Maturation and Defolliculation

7:18

Fertilization of In Vitro-cultured Oocytes

8:15

Results: Maternal Gene Product Manipulation Using In Vitro Oocyte Maturation

9:36

Conclusion

Transcript

An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.

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