Results: Chromosome Segregation, Spindle Length, and Acetylation of Oocytes Matured In Vitro and In Vivo
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Conclusion
Transcript
The overall goals of this experimental approach are to, first, morphologically and biochemically characterize horse oocytes and, second, to compare gametes from the same animal that were processed in vitro or in vivo. This experimental approach ca
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This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.