Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55255-v
May 24th, 2017
Chapters
0:05
Title
10:50
Conclusion
9:13
The SPLASH Workflow and Representative Results
1:12
Treatment of HeLa Cells with Biotinylated Psoralen RNA Extraction, and Fragmentation
2:25
RNA Size Selection and Elution
4:41
Enrichment of RNA Crosslinking Regions
5:57
Proximity Ligation
7:34
Reverse Crosslinking of Biotinylated Psoralen, and Amplification of cDNA
在这里,我们详细介绍了P soralen交联,Ligated和S选择的H ybrids(SPLASH)的S等位基因的方法,使得能够在体内对分子内和分子间RNA-RNA相互作用进行基因组范围的映射。 SPLASH可用于研究包括酵母,细菌和人在内的生物体的RNA相互作用。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved