Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization

0:51

Transfer of hiPSCs Grown on iMEF Feeder Cells onto Extracellular Matrix for Propagation

2:56

Small-scale Passaging of hiPSCs on Matrix-coated Plates

4:05

Preparation of Chamber Slides and Coverslips for Seeding of hiPSCs for Immunocytochemistry

6:04

Immunostaining of hiPSCs with Pluripotency Markers

8:12

Results: Representative Images of iPSC Colonies and Immunocytochemical Characterization of Human iPSCs

9:10

Conclusion