THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
3:54
Cytotoxicity Analysis Using an MTT Assay
6:06
In-broth Activity Analysis Using a Resazurin Assay
7:16
Results: Efficacy Screening of Inhibitors of M. tuberculosis
8:53
Conclusion
Transcript
The overall goal of this protocol is to provide multidimensional assessment of how compounds affect M.tuberculosis survival inside human macrophages. This method is centered on a Luciferase-based efficacy assay. When combined with cytotoxicity an
Sign in or start your free trial to access this content
We have developed a modular high-throughput screening system for discovering novel compounds against Mycobacterium tuberculosis, targeting intracellular and in-broth growing bacteria as well as cytotoxicity against the mammalian host cell.