THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
3:54
Cytotoxicity Analysis Using an MTT Assay
6:06
In-broth Activity Analysis Using a Resazurin Assay
7:16
Results: Efficacy Screening of Inhibitors of M. tuberculosis
8:53
Conclusion
Transcript
The overall goal of this protocol is to provide multidimensional assessment of how compounds affect M.tuberculosis survival inside human macrophages. This method is centered on a Luciferase-based efficacy assay. When combined with cytotoxicity an
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Vi har utvecklat ett modulärt högeffektiv screening-system för att upptäcka nya föreningar mot Mycobacterium tuberculosis, targeting intracellulära och i-buljong växande bakterier samt cytotoxicitet mot däggdjursvärdcellen.