Embryo Microinjection and Spontaneous Coiling Analysis
5:50
Imaging Setup for Mermaid Biosensor Visualization in Living Embryos: Simulataneous Detection of Donor and Acceptor Signals
8:23
Results: In Vivo FRET Analysis to Measure Changes in Motor Neuron Membrane Potential
9:39
Conclusion
Transcript
The overall goal of this procedure is to study the electrical properties of excitable cells in noninvasive physiological conditions employing zebrafish embryos together with a FRET-based GEVI. This method can help answer key questions concerning t
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Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.