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JoVE Journal

Developmental Biology

This content is Open Access.

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A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

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07:59 min

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May 17th, 2017

May 17th, 2017

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9,826 Views

0:05

Title

0:50

Preparing Seedling Growth Imaging Chambers

4:01

Using the Imaging Chambers

5:40

Results: Root Growth in Chambers Compared to Agar Plates

7:15

Conclusion

Transcript

Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

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