Preparation and slicing with a vibratome. Three agar blocks are needed to stabilize the brain during the slicing procedure. To avoid contamination, use an Autoclave set of instruments.
Remove the agar. Should be dried on the filter paper bef
Sign in or start your free trial to access this content
Organotypic hippocampus skive kulturer (OHSC) representerer en i vitro modell som simulerer i vivo situasjonen veldig godt. Her beskriver vi en vibratome-basert forbedret kutting protokoll for å få høy kvalitet skiver for bruk i vurderingen neuroprotective potensialet i romanen stoffer eller biologisk atferd av kreftceller.