Name: maturation of human stem cell-derived cardiomyocytes in biowires using electrical stimulation
Uploaded: 2017-05-06T13:00:00
Description: Watch this Scientific Journal Video about Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation at JoVE.com

这项工作是由一个赠款的援助从心脏和中风支持加拿大（G-14-0006265）的基础，从健康研究（137352和143066）和普·比克尔基金会授予加拿大学院运营赠款（1013821 ）到SSN。

主设计与制作注意：使用软光刻技术进行器件制造。为二甲基硅氧烷（PDMS）成型制成两层SU-8主机。 <ol><li>使用设计和绘图软件设计设备（ 图1A ，左）。分别绘制主体的每一层。在20,000 dpi的两个光掩模上打印设备设计，对应于主板15的两层。将设备图案设置为透明，周围区域为黑色;掩模将用作通过光刻将器件图案光学转移到SU-8上的模板（如其...

<ol>
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该手稿描述了改进hPSC-CMs成熟的设计平台的生物线的设置和实现。该装置可以在标准的微细加工设备中制造，并且生物线可以用普通的细胞培养技术和电刺激器生产。 据我们所知，没有报道类似于生物线的方法。该策略表明，改善的成熟性质取决于电刺激方案，如通过较高刺激速率上升方案（6Hz对3Hz）的生物线中获得的较大成熟水平所证明的。生物线中的心肌细胞与培养20天?...

细胞治疗是最有前途和研究战略，以实现心肌修复/再生一个。已经通过心脏组织工程和生物材料1，2的共递送辅助。大多数现有的细胞来源进行了研究，在动物模型为他们的损伤，患病或年老心3潜在的有利影响。尤其显著已经努力用人类多能干细胞（HPSC）衍生的心肌细胞（HPSC-CM），用于心脏组织工程可能无限的自体细胞来源。 HPSC-CMS可以使用多种已建立的方案4，5，6来制造。然而，所获得的细胞相比，成年心室肌7显示胚胎样表型，具有一定范围的未成熟的特性， </sup&gt; 8。这可能是一个障碍HPSC-CMS应用在药物研发和成人心脏疾病模型9的发展成人心脏组织的模型。 为了克服表型不成熟的这种限制，新方法已被积极地研究促进心肌细胞成熟。早期的研究通过循环机械10或电刺激11显露在新生大鼠心肌细胞的有效促成熟性质。凝胶压实和环状机械刺激还显示出改善HPSC-CM熟化12， 图13，与电生理和钙的处理性能的增强最小的某些方面。因此，所谓的“生物丝”（biowire）的平台系统是由同时提供结构线索和电场stimulatio设计n增强hPSC-CMs的成熟14 。该系统使用微制造平台来创建适合于电场刺激的对准的心脏组织。这可以用于改善hPSC-CM的结构和电生理成熟度。在这里，我们描述制作这样的生物线的细节。

<table border="0" cellpadding="0" cellspacing="0" width="582">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">L-Ascorbic acid</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">A-4544</td>
			<td style="width:167px;">hPSC-CM culture media componet</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">AutoCAD</td>
			<td style="width:80px;">Autodesk, Inc</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">Software to design device</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Carbon rods, &#216; 3 mm</td>
			<td style="width:80px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;">Electrical stimulator chamber component</td>
		</tr>
		<tr height="206">
			<td height="206" style="height:206px;width:216px;">Collagen, type 1, rat tail</td>
			<td style="width:80px;">BD Biosciences</td>
			<td style="width:119px;">354249</td>
			<td style="width:167px;">Collagen gel: 2.1 mg/ml of rat tail collagen type I in 24.9 mM glucose, 23.8 mM NaHCO3, 14.3 mM NaOH, 10 mM HEPES, in 1xM199 media with 10 % of growth factor-reduced Matrigel.</td>
		</tr>
		<tr height="164">
			<td height="164" style="height:164px;width:216px;">Collagenase type I&#160;</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">C0130</td>
			<td style="width:167px;">0.2% collagenase type I (w/v) and 20% FBS (v/v) in PBS with Ca2+ and Mg2+. Sterilize with 0.22 &#956;m filter and make 12 ml aliquots. Store at -20 &#176;C.</td>
		</tr>
		<tr height="100">
			<td height="100" style="height:100px;width:216px;">Deoxyribonuclease I (DNase I)</td>
			<td style="width:80px;">EMD Millipore</td>
			<td style="width:119px;">260913-25MU</td>
			<td style="width:167px;">Make 1 mg/ml DNase I stock solution in water. Filter sterile and store 0.5 ml aliquots at &#8722;20 &#176;C</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Drill &#38; drill bits (&#216; 1mm and 2 mm)</td>
			<td style="width:80px;">Dremel</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">Drill holes in polycarbonate frames</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Electrical stimulator</td>
			<td style="width:80px;">Grass</td>
			<td style="width:119px;">s88x</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Fetal bovine serum (FBS)</td>
			<td style="width:80px;">WISENT Inc.</td>
			<td style="width:119px;">080-450</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">D-(+)-Glucose&#160;</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">G5767</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">L-Glutamine</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">25030081</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:216px;">H2O</td>
			<td style="width:80px;">MilliQ</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">18.2 M&#937;&#183;cm at 25 &#176;C, ultrapure, to make all solutions</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">HEPES</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">H4034</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Hot plate</td>
			<td style="width:80px;">Torrey Pines</td>
			<td style="width:119px;">HS40</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Iscove&#39;s Modified Dulbecco&#39;s Medium(IMDM)</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">12440053</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Mask aligner</td>
			<td style="width:80px;">EVG&#160;</td>
			<td style="width:119px;">EVG 620</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Matrigel, growth factor reduced&#160;</td>
			<td style="width:80px;">Corning</td>
			<td style="width:119px;">354230</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Medium 199 (M199)</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">11150059</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Monothioglycerol (MTG)</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">M-6145</td>
			<td style="width:167px;">hPSC-CM culture media componet</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Orbital shaker</td>
			<td style="width:80px;">VWR</td>
			<td style="width:119px;">89032-088</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Penicillin/Streptomycin (P/S)</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">15070063</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Phosphate-buffered saline (PBS) with Ca2+ and Mg2+&#160;</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">14040133</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Plate (6-well)</td>
			<td style="width:80px;">Corning</td>
			<td style="width:119px;">353046</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Plate (6-well), low attachment</td>
			<td style="width:80px;">Corning</td>
			<td style="width:119px;">3471</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Platinum wires, 0.2 mm</td>
			<td style="width:80px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;">Electrical stimulator chamber component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Polydimethylsiloxane (PDMS)</td>
			<td style="width:80px;">Dow Corning</td>
			<td style="width:119px;">Sylgard 184</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Propylene glycol monomethyl ether acetate (PGMEA)</td>
			<td style="width:80px;">Doe &#38; Ingalls Inc.</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">To develop the wafer</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Pouch, peel-open</td>
			<td style="width:80px;">Convertors</td>
			<td style="width:119px;">92308</td>
			<td style="width:167px;">For steam sterilization</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Silicon wafer, 4-inch</td>
			<td style="width:80px;">UniversityWafer Inc.</td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="46">
			<td height="46" style="height:46px;width:216px;">Sodium bicarbonate (NaHCO3)</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">S5761</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Sodium hydroxide</td>
			<td style="width:80px;">Sigma</td>
			<td style="width:119px;">S8045</td>
			<td style="width:167px;">Collagen gel component</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Sprin coater</td>
			<td style="width:80px;">Specialty Coating Systems</td>
			<td style="width:119px;">G3P-8</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="280">
			<td height="280" style="height:280px;width:216px;">StemPro-34 culture medium</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">10639011</td>
			<td style="width:167px;">hPSC-CM culture medium. To make 50 ml, add 1.3 ml supplement, 500 &#956;l of 100&#215; L-Glutamine, 250 &#956;l of 30 mg/ml transferrin, 500 &#956;l of 5 mg/ml ascorbic acid, 150 &#956;l of 26 &#956;l /2 ml monothioglycerol (MTG), and 500 &#956;l (1 %) penicillin/streptomycin.</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Stop media</td>
			<td style="width:80px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;">&#160;Wash medium:FBS (1:1)</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">SU-8 50&#160;</td>
			<td style="width:80px;">MicroChem Corp.</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">photoresist, master component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">SU-8 2050&#160;</td>
			<td style="width:80px;">MicroChem Corp.</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">photoresist, master component</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">Transferrin</td>
			<td style="width:80px;">Roche</td>
			<td style="width:119px;">10-652-202</td>
			<td style="width:167px;">hPSC-CM culture media componet</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Trypsin/EDTA, 0.25%</td>
			<td style="width:80px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">25200056</td>
			<td style="width:167px;">hPSC-CM culture media componet</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:216px;">Wash medium</td>
			<td style="width:80px;"></td>
			<td style="width:119px;"></td>
			<td style="width:167px;">IMDM containing 1% Penicillin/Streptomycin</td>
		</tr>
	</tbody>
</table>

maturation of human stem cell-derived cardiomyocytes in biowires using electrical stimulation

人类多能干细胞衍生的心肌细胞（HPSC-CMS）是一个有前途的细胞来源，并从而鼓励他们在心脏研究潜力的应用，包括药物发现，疾病建模，组织工程和再生医学的研究。然而，由现有的协议产生的细胞显示与天然成年心室肌相比的范围不成熟。许多已作出努力以成熟HPSC-CMS，只有迄今达到中等成熟。因此，工程化的系统，称为biowire，已通过提供物理和电线索导致HPSC-CMS 在体外更加成熟的状态设计的。该系统使用微制造平台种子HPSC-CM的I型胶原沿着一个刚性模板缝合组装成对准的心脏组织（biowire），将其进行电场刺激具有逐渐增加的频率胶凝。相比未刺激控制，刺激biowired心肌细胞表现出的结构和电生理学成熟的增强程度。这样的变化是取决于刺激速率。这份手稿详细描述了biowires的设计和创作。

理性在biowires使用的缝合线的是，以作为在一个轴对准并模仿心肌纤维的形状的3D结构的形成的模板。我们发现，之后在biowire培养7天的细胞改造围绕缝合（ 图3A）的凝胶。沿着缝合线的轴线组装细胞形成对准心脏组织（ 图3）。后7天的预培养物，在biowires进行7天电场刺激的，并与心肌细胞的结构和功能的成熟兼容进一步表现出的性质。 <p class="...

Watch this Scientific Journal Video about Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation at JoVE.com

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

心脏biowire平台是使用三维细胞培养与电刺激结合成熟人胚胎和诱导的多能干细胞衍生的心肌细胞（HPSC-CM）的体外方法。这份手稿介绍了心脏biowire平台的详细设置。

使用电刺激人干细胞衍生的心肌细胞的成熟中Biowires

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have been a promising cell source and have thus encouraged the investigation of their ...

The overall goal of this procedure is to utilize controlled three dimensional cell cultivation methods in electrical stimulation to promote the maturation of human embryonic and induced pluripotent stem cells derived cardiomyocytes. This method can help answer key questions in the cardiac tissue engineering field including how different electrical stimulation parameters or length of time affect cardiac cell function and maturation state. The end results are engineered human cardiac tissues of improved maturity.The implications of this technique extend toward diagnosis applications such as testing the safety and efficacy of new drugs because cardiomyocyte with mature characteristics such as electrical progress are most likely to serve as more accurate models of human cardiac cells in drug screening studies. Combine the PDMS monomer and curing agent at a 10:1 ratio and mix well. Let it set for five minutes to degas.Then load a Petri dish containing the master for the biowire template with the PDMS mixture. Try to minimize air bubble formation. Next cure the PDMS at 70 degree Celsius for two hours.Then recover the solidified mold by first using a sharp blade around the edges and then peeling it away. Then trim the excess PDMS and collect all the templates in a sterilization pouch. Steam auto clave the templates at 121 degree Celsius for 20 minutes.And then load each template into a sterile Petri dish under the ventilation of a biosafety cabinet. Now for each template use tweezers to mount a piece of sterile 6.0 silk suture into the groove spanning the entire channel. The templates are then ready for cell seeding.The suture will serve as a template for the formation of 3D constructs that align along the suture axis mimicking the shape of cardiac fibers. For each chamber, make a pair of rectangular polycarbonate pieces to serve as the frame for the electrical stimulation chamber. Along the center line of each piece, drill two three millimeter wide holes, two centimeters apart.For each chamber prepare two 1.5 centimeter long carbon rods. About 3 millimeters from the end of each rod drill a one millimeter wide hole. Then thread each hole with a platinum wire and wrap one end of the wire around the rod.Next, for each chamber insert two rods between two polycarbonate pieces making a frame. Then pour about two milliliters of PDMS into a well of a six well plate and place the frame into the well with PDMS, submerging the bottom of the frames. Cure the PDMS at 70 degree Celsius for two hours so the PDMS secures the frame.Then remove the completed chamber from the plate with the PDMS attached. To test the function of an assembled chamber connect the platinum wires to a battery and use a voltmeter to check that electricity is being passed through the rods. Now transfer the functional chambers into a sterilization pouch and use 20 minutes of steam sterilization at 121 degree celsius to prepare them for cell culture.To prepare the gel, precool all the gel components on ice before mixing them in a sterile tube. Make sure to add the collagen and extra cellular matrix last and mix the components using pipetting. For the cells have cardiomyocytes differentiated from human pluripotent stem cells.Collect the cells and for each biowire preparation plant prepare 3.5 microliters of collagen gel containing half a million cells. Now use a P10 pipette tip to load approximately four milliliters of suspension into the 0.5 centimeter long channel template in each dish. Adjust the position of the suture using sterile forceps as needed so it is fully stretched.Once seeding the cells, make sure to always resuspend the gel cell mixture before taking autoclave in order to have consistent results. After loading the channels, cover the bottom of the dish with sterile PBS making sure not to touch the gel. This will ensure that the biowires do not dehydrate during polymerization.After incubating the biowires at 37 degree celsius for 30 minutes, replace the PBS with enough culture medium to cover the cells. 12 milliliters is usually enough. Then culture the dish for a week replacing the medium every other day.In a bio safety cabinet add two milliliters of PBS to each well of a six well plate that will house an electrical stimulator chamber. Then use sterile tweezers to gently place the autoclave devices into the wells. Once in position, discard the PBS and replace it with five milliliters of warm medium which should completely cover the carbon rods.Before culturing the cells for 24 hours, observe the cardiomyocytes contractility in response to electrical stimulation to ensure that the cells are capturing the electrical stimulus and responding with contractions. Next, place the biowires into the electrical stimulator chamber and orient the channels perpendicularly to the carbon rods. Extend the platinum wires away from the plate and then cover the plates.Connect the wires to an electrical stimulator. Set the stimulator to deliver biphasic one millisecond pulses at one hertz and at three volts per centimeter. Then transfer the preparations in the electrical stimulator to an incubator at 37 degree celsius.Increase stimulation rate incrementally every 24 hours from one hertz at day zero to six hertz at day six. During this week of culture, change the medium every other day. And observe the cardiomyocytes contractility on a daily basis.After seven days of preculture in the biowire cells remolded the gel around the suture. The cells assembled along the axis of the suture to form aligned cardiac tissue. The cells expressed cardiac contractile proteins showed sarcomeric banding in the contractile apparatus and their myofibrils were aligned along the axis of the suture.Cells in the biowire exhibited lower proliferation rates than cells in embryoid bodies. Calcium handling was significantly improved in the cardiomyocytes cultivated in biowires. Treatment with caffeine induced an abrupt release of calcium from the sacroplasmic reticulum.Such transients were found only in stimulated cells. There were also significantly higher stimulation frequency depending calcium release amplitudes. In addition, cardiomyocytes cultured in biowires showed improved hERG and inward rectifier current densities compared to cultivation in embryoid bodies which was further enhanced by electrical stimulation.The improved inward rectifier current was consistent with increased expression of the potassium inwardly rectifying channel jean, KCN J2.Automaticity the ability to beat spontaneously was significantly higher in embryoid body derived cardiomyocytes compared to controlled biowires. Control showed automaticity comparable to biowire preparation subjected to the six hertz regimen. Once mastered the setup of the biowires can be done in half a day.The setup of electrical stimulation will take a couple of hours and the final results can be reached in two weeks. While attempting this procedure, it is important to remember that cardiomyocytes differentiated from different cell lines or by different protocols can vary slightly in their maturity and purity. Therefore optimizing the experimental parameters such as the stimulation rate to your cells might be required.Following this procedure, other conditions including variation of the cellular composition and the utilization of mutant cardiomyocytes can be optimized to answer a bevy of additional questions.

Research

JoVE Journal

Developmental Biology

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

从人类诱导多能干细胞使用小分子调制分化和随后的葡萄糖饥饿高度纯化心肌推导

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have become an important cell source to address the lack of primary cardiomyocytes ...

科研

发育生物学

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

转基因器官皮肤培养使用灭活人真皮代

Organotypic cultures allow the reconstitution of a 3D environment critical for cell-cell contact and cell-matrix interactions which mimics the function ...

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

从多能干细胞使用BMP拮抗剂Grem2心房心肌细胞分化

Protocols for generating populations of cardiomyocytes from pluripotent stem cells have been developed, but these generally yield cells of mixed ...

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产

Maximizing the benefit of human pluripotent stem cells (hPSCs) for research, disease modeling, pharmaceutical and clinical applications requires robust ...

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

多能干细胞衍生的心肌修复心肌细胞

Human induced pluripotent stem cells (hiPSCs) must be fully differentiated into specific cell types before administration, but conventional protocols for ...

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

人多能干细胞对原始和最终造血祖细胞定向分化的研究

One of the major goals for regenerative medicine is the generation and maintenance of hematopoietic stem cells (HSCs) derived from human pluripotent stem ...

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

产妇基因产品的官能化操作使用<em&gt;体外</em斑马鱼&gt;卵母细胞成熟

Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into ...

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs

使用多电极阵列（MEA）的人多能干细胞来源的心肌细胞（hPSC-CM）的电生理分析

Cardiomyocytes can now be derived with high efficiency from both human embryonic and human induced-Pluripotent Stem Cells (hPSC). hPSC-derived ...

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

人诱导多潜能干细胞衍生肝细胞的药物发现方法

The ability to differentiate human induced pluripotent stem cells (iPSCs) into hepatocyte-like cells (HLCs) provides new opportunities to study inborn ...

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

诱导多潜能干细胞源性肝细胞的家族性高胆固醇血症人肝嵌合小鼠模型

Familial hypercholesterolemia (FH) is mostly caused by low-density lipoprotein receptor (LDLR) mutations and results in an increased risk of early-onset ...