Methodology: Second Subculturing, Cells Detaching and Counting
7:45
Methodology: Antibody Quantitation and Dilutions
9:37
Methodology: Neutralization Assay
13:00
Methodology: Spectrophotometric Analysis
13:54
Results: Dose Response Curve
15:23
Conclusion
Transcript
The overall goal of this procedure is to measure the relative potency of anti-TNF-alpha molecules. In this example, we compare two anti-TNF-alpha monoclonal antibodies in order to determine the neutralization efficiency of one reference mAb versus
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A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.