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JoVE Journal

Neuroscience

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Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

11.6K Views

10:30 min

February 15th, 2017

February 15th, 2017

11,631 Views

0:05

Title

1:11

Brain Slice and Retinal Wholemount Preparation

4:02

Calcium Dye Loading

5:38

Electrophysiological Recordings and Imaging

7:28

Results: Electrophysiological and Calcium Recordings in Retinal Wholemount

8:49

Conclusion

Transcript

The overall goal of this technique is to maintain neuronal tissue for prolonged periods without significant tissue degradation. Using this method, electrophysiological recordings and calcium imaging can be conducted more than 24 hours after tissue

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Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.

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