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DOI :
10.3791/55411-v
March 7th, 2017
Chapters
0:05
Title
0:59
Introducing the Wounder Parts
1:38
Setting the Pin Holder Width
2:05
Adjusting the Guiding Bars
2:49
Calibrating the Pins
3:23
Scratching the Cell Monolayer
4:44
Results: Uniform Wound Width with Device
5:13
Conclusion
ここでは、96ウェル細胞培養プレートに半ハイスループット細胞遊走アッセイを実行するためのプロトコルを提示します。このプロトコルは、細胞単層上で一貫性のあるスクラッチ傷を作成するには、高速、シンプルかつ経済的な方法です。
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