Evaluación de la Viabilidad Neuronal Utilizando Colorante de Fluoresceína-Ioduro de Propidio Doble Coloración en Gránulos Cerebelosos Cultura de Neuronas
Results: Double and Triple Stainings in CGN Culture
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Tissue Harvest from 8 Day Old Sprague-Dawley Rats
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FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
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Assessment of Neuronal Viability
Transcript
The overall goal of this procedure is to accurately measure cell viability in a cerebellar granule neuron culture. This method can help to answer key questions in the field of neuroscience and neuropharmacology. The main advantage of this techniqu
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Este protocolo describe cómo medir con precisión la viabilidad neuronal utilizando fluoresceína diacetato (FDA) y Propidium Iodide (PI) doble tinción en neuronas cultivadas de gránulos cerebelosos, un cultivo neuronal primario utilizado como un modelo in vitro en neurociencia y neurofarmacología investigación.