Results: Double and Triple Stainings in CGN Culture
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Tissue Harvest from 8 Day Old Sprague-Dawley Rats
3:51
FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
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Assessment of Neuronal Viability
Transcript
The overall goal of this procedure is to accurately measure cell viability in a cerebellar granule neuron culture. This method can help to answer key questions in the field of neuroscience and neuropharmacology. The main advantage of this techniqu
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Detta protokoll beskriver hur man noggrant mäter neuronal lönsamhet med hjälp av fluoresceindiacetat (FDA) och propidiumjodid (PI) dubbelfärgning i odlade cerebellära granulärneuroner, en primär neuronkultur som används som in vitro- modell vid neurovetenskap och neurofarmakologiforskning.