Análisis de terminación de la transcripción Uso Enfoque BrUTP-capítulo específico de la transcripción de ejecución en (TRO)

We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.