Results: Effect of Saquinavir on Primary Human Fibroblasts
0:47
Seeding Cells in a 96-well Plate
1:48
Setting Up the Microscope and Acquisition Protocol
3:10
Loading of the Cells with CM-H2DCFDA and TMRM
4:18
First and Second Live Imaging Rounds
6:22
Image Processing and Analysis
Transcript
The overall goal of this high-content microscopy-based method is to simultaneously quantify mitochondrial morphofunction and cellular levels of reactive oxygen species so as to establish a robust redox fingerprint for cell lines exposed to differe
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I dette papiret presenteres en mikroprosess arbeidsflyt med høy innhold for samtidig kvantifisering av intracellulære ROS-nivåer, samt mitokondrielt membranpotensial og morfologi - i fellesskap referert til som mitokondriell morfofunksjon - i levende vedhengende celler ved bruk av celle-permeant fluorescerende reportermolekyler 5- (og- 6) -klorometyl-2 ', 7'-diklorodihydrofluoresceindiacetat, acetylester (CM-H2 DCFDA) og tetrametylrhodamin-metylester (TMRM).