Infection Protocol to Generate Mtb/Macrophage Aggregate Structures
4:04
Growth Inhibition Assay to Assess Drug Efficacy against Mtb Derived from Mtb/Macrophage Aggregates
6:31
Quantification of Drug Efficacy Using the Resazurin Microtiter Assay
7:54
Using the Resazurin Microtiter Assay to Measure Mtb Viability
9:52
Conclusion
Transcript
The overall goal of this procedure is to reproducibly generate mycobacterium tuberculosis infected macrophage aggregate structures in 96 well plate format for drug susceptibility testing using a viability dye. This method can improve the early dru
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New models and assays that would improve the early drug development process for next-generation anti-tuberculosis drugs are highly desirable. Here, we describe a quick, inexpensive, and BSL-2 compatible assay to evaluate drug efficacy against Mycobacterium tuberculosis that can be easily adapted for high-throughput screening.