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JoVE Journal

Immunology and Infection

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A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

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10:29 min

March 24th, 2017

March 24th, 2017

7,679 Views

0:05

Title

0:46

Infection Protocol to Generate Mtb/Macrophage Aggregate Structures

4:04

Growth Inhibition Assay to Assess Drug Efficacy against Mtb Derived from Mtb/Macrophage Aggregates

6:31

Quantification of Drug Efficacy Using the Resazurin Microtiter Assay

7:54

Using the Resazurin Microtiter Assay to Measure Mtb Viability

9:52

Conclusion

Transcript

The overall goal of this procedure is to reproducibly generate mycobacterium tuberculosis infected macrophage aggregate structures in 96 well plate format for drug susceptibility testing using a viability dye. This method can improve the early dru

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New models and assays that would improve the early drug development process for next-generation anti-tuberculosis drugs are highly desirable. Here, we describe a quick, inexpensive, and BSL-2 compatible assay to evaluate drug efficacy against Mycobacterium tuberculosis that can be easily adapted for high-throughput screening.

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