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DOI :
10.3791/55498-v
•
9:35 min
April 1st, 2017
Chapters
0:05
Title
0:40
Isolation of Native Protein Complexes by FLAG Affinity Purification
3:10
Blue Native PAGE
4:39
Preparation for Mass Spectrometry
8:06
Results: Characterization of Two Distinct Mta2-containing NuRD Complexes by ABC-MS
8:54
Conclusion
ここでは、ラベルフリーの定量的質量分析法を用いたタンパク質相関プロファイリングに続く親和性タンパク質複合体の精製と青ネイティブPAGEによるそれらの分離のためのプロトコルを提示します。この方法は、異なるタンパク質複合体へのインタラクトームを解決するのに便利です。
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