Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
10:30
CFSE Labeling of Responder Cells to Measure their Proliferation
12:09
FACS Staining and Analysis After 6 Days of Coculture
13:49
In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33
Representative Results
18:03
Conclusion
Transcript
Tolerance induction in transplantation is often associated with emergence of regulatory cell population. Absence of immune response to the donor, but preserved immunity against exogenous antigen. We have different protocol to assess in vitro and i
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Här presenterar vi ett protokoll för att inducera tolerans vid transplantation, och bedöma in vitro och i vivo immunstatus hos mottagaren mot givaren eller exogena antigen och distinkta cell delmängder av mottagaren suppressiv kapacitet.