Name: exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal
Uploaded: 2017-04-13T14:00:00
Description: Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Forfatterne anerkender med taknemmelighed den dygtige bistand fra fru Trine W. Mikkelsen, fru Lise M. Fitting, og personalet på Påskehøjgaard. Den danske Sundhedsvidenskabelige Forskningsråd, Lundbeckfonden og Novo Nordisk Fonden støttes økonomisk undersøgelsen.

Animal anæstesi og euthanesia blev udført i overensstemmelse med &quot;Principper for laboratoriedyr pleje&quot; (NIH publikation nr 86-23, revideret 1985) og godkendt af Dansk Råd for Animal Research Ethics. 1. instrumenter <ol><li> Saml de instrumenter, der præsenteres i videoen og opført i tabellen of Materials. </li></ol> 2. Decapitation BEMÆRK: Anæstesi blev induceret ved en intramuskulær injektion af 5 ml af ...

<ol>
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R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypothalamic+deep+brain+stimulation+influences+autonomic+and+limbic+circuitry+involved+in+the+regulation+of+aggression+and+cardiocerebrovascular+control+in+the+minipig.">Hypothalamic deep brain stimulation influences autonomic and limbic circuitry involved in the regulation of aggression and cardiocerebrovascular control in the minipig.</a> Stereotact Funct Neurosurg. 90 (5), 281-291 (2012).</li><li>Nielsen, M. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Continuous+MPTP+intoxication+in+the+minipig+results+in+chronic+parkinsonian+deficits.">Continuous MPTP intoxication in the minipig results in chronic parkinsonian deficits.</a> Acta Neurobiol Exp. 76, 198-210 (2016).</li><li>Bjarkam, C. 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Fleste eksperimentelle neurovidenskab undersøgelser udføres i små dyrearter, såsom mus og rotter, hvor adgang til CNS lettes af en tynd skull- og dural-tykkelse. Men i større forsøgsdyr som grise 1, 4, 8, får 32, og ikke-humane primater, den betydelige tykkelse af disse strukturer nødvendiggør anvendelsen af robuste instrumenter (table af materialer) og ordentlige indgange til kraniet fjernelse ...

<html> Translationelle neurovidenskab studier i svin er blevet mere og mere populær i løbet af de sidste to årtier. Den store størrelse af svinehjerne muliggør anvendelse af konventionelle kliniske hjerne kameraer og direkte anvendelse og test af neurokirurgiske procedurer og udstyr fra den humane klinik 1, 2, 3, 4, 5, 6, 7, 8. I de sidste 20 år, svin, især minigrise (fx, Gottingen minigrise), er blevet anvendt til at undersøge neuromodulatory behandlingsmodaliteter, såsom stamcelletransplantation; virusvektor transfektion; og dybhjemestimulation rettet mod Parkinsons sygdom, fedme, depression og Alzheimers sygdom 2, 6,= &quot;xref&quot;&gt; 9, 10, 11, 12, 13, 14, 15, 16, 17. Dette er blevet fulgt af udviklingen af stereotaksiske og kirurgiske metoder til at manipulere smågris CNS 3, 18, 19, 20, 21. De anlagt CNS ændringer er blevet evalueret i levende dyr ved anvendelse brain imaging (PET 10, 13, 22, 24 og MR 23), cystometri 11, 12, 25, ganganalyse17, neurologisk evaluering 9, 17, og obduktion baseret på histologi og stereologiske analyse 14, 15, 17, 26, 27, 31. Imidlertid post mortem analyse kræver eksponering og fjernelse af svinehjerne, som ikke er en let opgave, som en tyk, benet kranium og et fibrøst dural dækker omgiver svinehjerne. Målet med dette papir og instruktions video er at beskrive, hvordan den postmortem svinehjerne og hypofyse kan eksponeres og fjernes i en intakt tilstand i 15-20 min under anvendelse af ikke-motoriserede kirurgiske redskaber. Den instruktionsvideo og fotografiske illustrationer viser mandlige minigrise (alder: 6 måneder, legemsvægt: 20-25 kg), der anvendes til en anatomisk undersøgelse minigrise hypofysen.

<table border="0" cellpadding="0" cellspacing="0" width="1036">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Heavy Scalpel Handle #4</td>
			<td style="width:191px;">FST (Fine Science Tools)</td>
			<td style="width:149px;">10008-13</td>
			<td style="width:481px;">Good for skin incision and soft tissue removal</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #23</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10023-00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Scalpel Handle #7</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10007-12</td>
			<td>Optimal for dural incision and precision work</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #11</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10011-00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical Forceps</td>
			<td style="width:191px;">FST&#160;</td>
			<td>11024-18</td>
			<td>The tip of the surgical forceps ensure a firm grip&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Kerrison Bone Punch</td>
			<td style="width:191px;">Aesculap Neurosurgery</td>
			<td style="width:149px;">FF713R</td>
			<td>Must be robust, bite size 3-5 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">MD615</td>
			<td>Must be robust, bite size 15 x 5 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">FO551R</td>
			<td>Must be robust, bite size 25 x 15 mm&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bone Chisel</td>
			<td>Lawton</td>
			<td>67-0335</td>
			<td>The size of the chisel head should not exceed 20 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mallet (Hammer)</td>
			<td>Millarco</td>
			<td>5624108</td>
			<td>Weigth 300 g, length 30 cm, head hit area size 2 x 2 cm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro-Scissor</td>
			<td>FST&#160;</td>
			<td>14002-14&#160;&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dissector</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">OL165R</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">&#160;G&#246;ttingen minipigs</td>
			<td style="width:191px;">&#160;Ellegaard G&#246;ttingen Minipigs A/S, Denmark</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Euthanimal</td>
			<td style="width:191px;"></td>
			<td style="width:149px;"></td>
			<td>pentobarbital</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Ketamine</td>
			<td style="width:191px;">Pfizer</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Midazolam&#160;</td>
			<td style="width:191px;">Hameln Pharmaceuticals</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal

Grise er blevet stadig mere populært i store dyr translationel neurovidenskab forskning som en økonomisk og etisk realistisk alternativ til ikke-menneskelige primater. Den store hjerne størrelse grisen tillader anvendelsen af ​​konventionelle kliniske hjerne kameraer og direkte anvendelse og test af neurokirurgiske procedurer og udstyr fra den humane klinik. Yderligere makroskopisk og histologisk analyse kræver imidlertid post mortem eksponering af grisen centralnervesystemet (CNS) og efterfølgende hjerne fjernelse. Dette er ikke en let opgave, som grisen CNS er indkapslet af en tyk, benet kranium og rygsøjle. Målet med dette papir og instruktions video er at beskrive, hvordan at blotlægge og fjerne den post mortem svinehjerne og hypofysen i en intakt tilstand, egnet til efterfølgende makroskopisk og histologisk analyse.

At forhindre vævet materiale tørrer ud, anbefales det at opbevare den fjernede hjerne og hypofyse i en krukke fyldt med fiksativ eller isotonisk saltvand umiddelbart efter at der er udført makroskopisk analyse. Vævet materiale kan opbevares i fiksativ i år, mens opbevaring i isotonisk saltopløsning, selv i et køleskab, vil føre til væv henfald med tiden. Den fjernede hypofyse kan også fryses direkte ved nedsænkning ...

Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Målet med dette papir og instruktions video er at beskrive, hvordan at blotlægge og fjerne den post mortem svinehjerne og hypofysen i en intakt tilstand, egnet til efterfølgende makroskopisk og histologisk analyse.

Eksponering af Pig CNS til histologisk analyse: En Manual til Decapitation, Skull Åbning og Brain Removal

Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to ...

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