Name: exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal
Uploaded: 2017-04-13T14:00:00
Description: Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Les auteurs reconnaissent avec gratitude l&#39;aide habile de Mme Trine W. Mikkelsen, Mme Lise M. Fitting, et le personnel Påskehøjgaard. Le Conseil de recherches médicales du Danemark, la Fondation Lundbeck et la Novo Nordisk Fondation a soutenu financièrement l&#39;étude.

anesthésie animale et euthanesia a été réalisée conformément aux « Principes de protection des animaux de laboratoire » (publication NIH n ° 86-23, révisé 1985) et approuvé par le Conseil danois pour l&#39;éthique de la recherche animale. 1. Instruments <ol><li> Recueillir les instruments présentés dans la vidéo et figurant dans la table des matières. </li></ol> 2. Décapitation NOTE: L&#39;anesthésie a ?...

<ol>
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N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+MRI-guided+stereotaxic+viral+mediated+gene+transfer+of+alpha-synuclein+in+the+minipig+CNS.">Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the minipig CNS.</a> Acta Neurobiol Exp. 71 (4), 508-518 (2011).</li><li>Ettrup, K. S., S&#248;rensen, J. C., Rodell, A., Alstrup, A. K. O., Bjarkam, C. 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S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basic+Surgical+Techniques+in+the+Minipig:+Intubation,+Transurethral+Bladder+Catheterization,+Femoral+Vessel+Catheterization,+and+Transcardial+Perfusion.">Basic Surgical Techniques in the Minipig: Intubation, Transurethral Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion.</a> J Vis Exp. (52), e2652 (2011).</li><li>Ettrup, K. S., Torn&#248;e, J., S&#248;rensen, J. C., Bjarkam, C. 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La plupart des études de neuroscience expérimentales sont réalisées en petites espèces d&#39;animaux, comme les souris et les rats, où l&#39;accès au SNC est facilitée par une fine épaisseur dural et skull-. Cependant, chez les animaux expérimentaux plus grands comme les porcs 1, 4, 8, les moutons 32 et les primates non humains, l&#39;épaisseur considérable de ces structures nécessite l&#39;...

<html> Des études en neurosciences translationnelles chez les porcs sont devenus de plus en plus populaires au cours des deux dernières décennies. La grande taille du cerveau de porc permet l&#39;utilisation d&#39;imageurs du cerveau clinique classiques et l&#39;utilisation directe et les tests des procédures neurochirurgicales et de l&#39; équipement de la clinique humaine 1, 2, 3, 4, 5, 6, 7, 8. Au cours des 20 dernières années, les porcs, en particulier miniporcs (par exemple, Göttingen minipig), ont été utilisées pour examiner les modalités de traitement neuromodulateurs, comme la transplantation de cellules souches; transfection de vecteur viral; et la stimulation cérébrale profonde dirigée vers la maladie de Parkinson, l&#39; obésité, la dépression, la maladie d&#39; Alzheimer et 2, 6,= &quot;xref&quot;&gt; 9, 10, 11, 12, 13, 14, 15, 16, 17. Cela a été suivi par le développement d&#39;approches chirurgicales stéréotaxiques et de manipuler le système nerveux central minipig 3, 18, 19, 20, 21. Les modifications du SNC instituées ont été évaluées chez des animaux vivants en utilisant l&#39; imagerie du cerveau (PET 10, 13, 22, 24 et 23 MR), cystometry 11, 12, 25, analyse de la marche17, l&#39; évaluation neurologique 9, 17, et examen post - mortem sur la base de l&#39; histologie et analyse stéréologique 14, 15, 17, 26, 27, 31. Cependant, l&#39;analyse post-mortem nécessite l&#39;exposition et l&#39;élimination du cerveau de porc, ce qui est pas une tâche facile, comme un crâne épais, osseux et un dural fibreux couvrant entourent le cerveau de porc. Le but de cet article et vidéo d&#39;instruction est de décrire comment le cerveau de porc post-mortem et hypophyse peuvent être exposés et éliminés dans un état intact 15-20 min à l&#39;aide des outils chirurgicaux non motorisés. La vidéo pédagogique et illustrations photographiques montrent miniporcs mâles (âge: 6 mois, poids vif: 20-25 kg) utilisés pour une étude anatomique sur l&#39;hypophyse minipig.

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			<td height="21" style="height:21px;width:216px;">Heavy Scalpel Handle #4</td>
			<td style="width:191px;">FST (Fine Science Tools)</td>
			<td style="width:149px;">10008-13</td>
			<td style="width:481px;">Good for skin incision and soft tissue removal</td>
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			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #23</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10023-00</td>
			<td></td>
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			<td height="21" style="height:21px;">Scalpel Handle #7</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10007-12</td>
			<td>Optimal for dural incision and precision work</td>
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		<tr height="21">
			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #11</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10011-00</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Surgical Forceps</td>
			<td style="width:191px;">FST&#160;</td>
			<td>11024-18</td>
			<td>The tip of the surgical forceps ensure a firm grip&#160;</td>
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			<td height="21" style="height:21px;">Kerrison Bone Punch</td>
			<td style="width:191px;">Aesculap Neurosurgery</td>
			<td style="width:149px;">FF713R</td>
			<td>Must be robust, bite size 3-5 mm</td>
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			<td height="21" style="height:21px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">MD615</td>
			<td>Must be robust, bite size 15 x 5 mm</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">FO551R</td>
			<td>Must be robust, bite size 25 x 15 mm&#160;</td>
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			<td height="21" style="height:21px;">Bone Chisel</td>
			<td>Lawton</td>
			<td>67-0335</td>
			<td>The size of the chisel head should not exceed 20 mm</td>
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			<td height="21" style="height:21px;">Mallet (Hammer)</td>
			<td>Millarco</td>
			<td>5624108</td>
			<td>Weigth 300 g, length 30 cm, head hit area size 2 x 2 cm</td>
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			<td height="21" style="height:21px;">Micro-Scissor</td>
			<td>FST&#160;</td>
			<td>14002-14&#160;&#160;</td>
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			<td height="21" style="height:21px;">Dissector</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">OL165R</td>
			<td></td>
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		<tr height="42">
			<td height="42" style="height:42px;">&#160;G&#246;ttingen minipigs</td>
			<td style="width:191px;">&#160;Ellegaard G&#246;ttingen Minipigs A/S, Denmark</td>
			<td style="width:149px;"></td>
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			<td height="21" style="height:21px;">Euthanimal</td>
			<td style="width:191px;"></td>
			<td style="width:149px;"></td>
			<td>pentobarbital</td>
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			<td height="21" style="height:21px;">Ketamine</td>
			<td style="width:191px;">Pfizer</td>
			<td style="width:149px;"></td>
			<td></td>
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			<td height="21" style="height:21px;">Midazolam&#160;</td>
			<td style="width:191px;">Hameln Pharmaceuticals</td>
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exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal

Les porcs sont devenus de plus en plus populaire dans la recherche en neurosciences translationnelle grand animal comme sur le plan économique et sur le plan éthique substitut possible aux primates non humains. La grande taille du cerveau du porc permet l&#39;utilisation des imageurs du cerveau clinique classique et l&#39;utilisation directe et le contrôle des procédures et de l&#39;équipement neurochirurgicales de la clinique humaine. Une analyse plus poussée macroscopique et histologique, cependant, exige une exposition post-mortem du système nerveux central de porc (CNS) et l&#39;élimination ultérieure du cerveau. Ce n&#39;est pas une tâche facile, comme le porc du système nerveux central est encapsulé par un crâne épais, osseux et de la colonne vertébrale. Le but de cet article et vidéo d&#39;instruction est de décrire comment exposer et enlever le cerveau de porc post-mortem et l&#39;hypophyse dans un état intact, adapté à l&#39;analyse macroscopique et histologique.

Pour éviter que le matériau de tissu de sécher, il est recommandé de stocker le cerveau enlevé et pituitaire dans un bocal rempli de fixatif ou une solution saline isotonique immédiatement après l&#39;analyse macroscopique a été réalisée. Le matériau de tissu peut être stocké dans le fixatif pendant des années, alors que le stockage dans une solution saline isotonique, même dans un réfrigérateur, conduira à la désintégration du tissu avec le temps. <p class="jove...

Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Le but de cet article et vidéo d&#39;instruction est de décrire comment exposer et enlever le cerveau de porc post-mortem et hypophyse dans un état intact, adapté à l&#39;analyse macroscopique et histologique.

L&#39;exposition du porc du système nerveux central pour l&#39;analyse anatomopathologique: Un manuel pour Décapitation, Crâne d&#39;ouverture et cerveau suppression

Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to ...

Research

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Anatomy and Physiology

Neuroscience

Unit 1

The Axial Skeleton

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A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Une poli et renforcé Diluée-crâne fenêtre for Long-term imagerie du cerveau de souris

In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form ...

Recherche

Neurosciences

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

Co-analyse de la structure du cerveau et son fonctionnement en utilisant l&#39;IRMf et imagerie de diffusion

The study of complex computational systems is facilitated by network maps, such as circuit diagrams. Such mapping is particularly informative when ...

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures

Un protocole complet pour la segmentation manuelle des structures du lobe temporal médial

The present paper describes a comprehensive protocol for manual tracing of the set of brain regions comprising the medial temporal lobe (MTL): amygdala, ...

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyse Synaptic Modulation de<em&gt; Drosophila melanogaster</em&gt; Photorécepteurs après exposition à la lumière prolongée

The nervous system has the remarkable ability to adapt and respond to various stimuli. This neural adjustment is largely achieved through plasticity at ...

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

Analyse de haut-débit du comportement locomoteur dans le dosage de l’île de drosophile

Advances in next-generation sequencing technologies contribute to the identification of (candidate) disease genes for movement disorders and other ...

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Enregistrement de la plasticité synaptique dans des tranches d’hippocampe aiguës maintenues dans un petit volume recyclage - Perfusion- et système de chambre de Submersion-type

Even though experiments on brain slices have been in use since 1951, problems remain that reduce the probability of achieving a stable and successful ...

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Roman passif méthodes de compensation pour la Production rapide de transparence optique dans tout le tissu CNS

Since the development of CLARITY, a bioelectrochemical clearing technique that allows for three-dimensional phenotype mapping within transparent tissues, ...

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

Implantation chronique d’ensemble-corticale Electrocorticographic tableau dans l’ouistiti commun

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

Ouverture de la barrière hémato-encéphalique induite par ultrasons focalisés pour cibler les structures cérébrales et évaluer la neuromodulation chimiogénétique

Acoustically Targeted Chemogenetics (ATAC) allows for the noninvasive control of specific neural circuits. ATAC achieves such control through a ...

Extraction and Dissection of the Domesticated Pig Brain

Extraction et dissection du cerveau de porc domestiqué

Use of the pig as a preclinical and translatable animal model has been well-documented and accepted by research fields investigating cardiovascular ...