Name: exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal
Uploaded: 2017-04-13T14:00:00
Description: Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Yazarlar minnettarlıkla Bayan Trine W. Mikkelsen ve usta yardımı, Montaj Bayan Lise M. ve Påskehøjgaard personeli kabul. Danimarkalı Tıbbi Araştırma Konseyi, Lundbeck Vakfı ve Novo Nordisk Vakfı maddi çalışmayı desteklemiştir.

Hayvan anestezi ve euthanesia (NIH yayın sayılı 86-23, 1985 revize) ve Hayvan Araştırmaları Etik Danimarka Konseyi tarafından onaylanan &quot;laboratuvar hayvan bakımı İlkeleri&quot; uyarınca gerçekleştirilmiştir. 1. Araçlar <ol><li> Enstrümanlarını videoda sunulan ve Malzemelerin Tablo listelenen toplayın. </li></ol> 2. Decapitation Not: Anestezi midazolam 5 mL (5 mg / mL) ve ketamin 5 ml (25 mg / ml) i...

<ol>
	<li>Lind, M. N., Moustgaard, A., Jelsing, J., Vajta, G., Cumming, P., Hansen, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+pigs+in+neuroscience:+Modeling+brain+disorders.">The use of pigs in neuroscience: Modeling brain disorders.</a> Neurosci Biobehav Rev. 31, 728-751 (2007).</li><li>Bjarkam, C. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuromodulation+in+a+minipig+model+of+Parkinson+disease.">Neuromodulation in a minipig model of Parkinson disease.</a> British J Neurosurg. 22 (Suppl. 1), S9-S12 (2008).</li><li>Bjarkam, C. R., Cancian, G., Glud, A. N., Ettrup, K. S., &#216;stergaard, L., S&#248;rensen, J. 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N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+MRI-guided+stereotaxic+viral+mediated+gene+transfer+of+alpha-synuclein+in+the+minipig+CNS.">Direct MRI-guided stereotaxic viral mediated gene transfer of alpha-synuclein in the minipig CNS.</a> Acta Neurobiol Exp. 71 (4), 508-518 (2011).</li><li>Ettrup, K. S., S&#248;rensen, J. C., Rodell, A., Alstrup, A. K. O., Bjarkam, C. 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S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basic+Surgical+Techniques+in+the+Minipig:+Intubation,+Transurethral+Bladder+Catheterization,+Femoral+Vessel+Catheterization,+and+Transcardial+Perfusion.">Basic Surgical Techniques in the Minipig: Intubation, Transurethral Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion.</a> J Vis Exp. (52), e2652 (2011).</li><li>Ettrup, K. S., Torn&#248;e, J., S&#248;rensen, J. C., Bjarkam, C. 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En çok deneysel nörolojik çalışmalar, CNS&#39;ye erişim ince skull- ve dural-kalınlığıyla kolaylaştırılmaktadır fareler ve sıçanlar gibi küçük hayvan türleri, gerçekleştirilir. Bununla birlikte, domuz, 1, 4, 8, koyun 32 ve insan olmayan primatlar gibi daha büyük bir deney hayvanlarında, bu yapıların önemli kalınlığı kafatası kemik çıkarılması için güçlü bir araçl...

<html> domuzlarda Translasyonal nörobilim çalışmaları son yirmi yılda giderek popüler hale gelmiştir. Domuz beyin büyük boyutlu klasik klinik beyin görüntüleyiciler ve doğrudan kullanımı ve insan klinik 1, 2, 3, 4, 5, 6, 7, 8 den nörocerrahi usul ve ekipman test kullanımını mümkün kılmaktadır. Son 20 yıl içinde, domuz, özellikle küçük domuzu (örneğin, Göttingen Minipig gibi), kök hücre transplantasyonu, nöromodülatör tedavi yöntemleri incelemek için kullanılmıştır; Viral vektör transfeksiyon; ve Parkinson hastalığı, obezite, depresyon ve Alzheimer hastalığı 2, 6 yönelik derin beyin uyarımı,= &quot;xref&quot;&gt; 9, 10, 11, 12, 13, 14, 15, 16, 17. Bu MiniPig MSS 3, 18, 19, 20, 21 işlemek için stereotaksik ve cerrahi yaklaşımların geliştirilmesi takip edilmiştir. Tesis MSS değişiklikleri (PET 10, 13, 22, 24 ve MR 23), sistometrisi 11, 12, 25, beyin görüntüleme kullanılarak canlı hayvanlarda değerlendirilmiştir, yürüme analizi17, nörolojik değerlendirme 9, 17 ve histoloji ve stereolojik analizi 14, 15, 17, 26, 27, 31 temel ölüm sonrası inceleme. Bununla birlikte, ölüm sonrası analizi kalın, kemik kafatası ve domuz beyin çevreleyen kapsayan lifli bir dural olarak maruz kalma ve kolay bir iş değildir domuz beyin, kaldırılmasını gerektirir. Bu kağıt ve öğretim videonun amacı postmortem domuz beyin ve hipofiz motorsuz cerrahi aletler kullanılarak 15-20 dakika içinde bozulmamış bir halde maruz ve kaldırılabilir nasıl tarif etmektir. MiniPig hipofiz bezi üzerinde anatomik bir çalışma için kullanılan öğretim video ve fotoğraf çizimler erkek domuzu (20-25 kg: 6 ay, vücut ağırlığı yaş) göstermektedir.

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			<td height="21" style="height:21px;width:216px;">Heavy Scalpel Handle #4</td>
			<td style="width:191px;">FST (Fine Science Tools)</td>
			<td style="width:149px;">10008-13</td>
			<td style="width:481px;">Good for skin incision and soft tissue removal</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #23</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10023-00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Scalpel Handle #7</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10007-12</td>
			<td>Optimal for dural incision and precision work</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Non-Sterile Scalpel Blades #11</td>
			<td style="width:191px;">FST&#160;</td>
			<td>10011-00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Surgical Forceps</td>
			<td style="width:191px;">FST&#160;</td>
			<td>11024-18</td>
			<td>The tip of the surgical forceps ensure a firm grip&#160;</td>
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		<tr height="21">
			<td height="21" style="height:21px;">Kerrison Bone Punch</td>
			<td style="width:191px;">Aesculap Neurosurgery</td>
			<td style="width:149px;">FF713R</td>
			<td>Must be robust, bite size 3-5 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">MD615</td>
			<td>Must be robust, bite size 15 x 5 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bone Rongeur</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">FO551R</td>
			<td>Must be robust, bite size 25 x 15 mm&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Bone Chisel</td>
			<td>Lawton</td>
			<td>67-0335</td>
			<td>The size of the chisel head should not exceed 20 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mallet (Hammer)</td>
			<td>Millarco</td>
			<td>5624108</td>
			<td>Weigth 300 g, length 30 cm, head hit area size 2 x 2 cm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro-Scissor</td>
			<td>FST&#160;</td>
			<td>14002-14&#160;&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dissector</td>
			<td>Aesculap Neurosurgery</td>
			<td style="width:149px;">OL165R</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">&#160;G&#246;ttingen minipigs</td>
			<td style="width:191px;">&#160;Ellegaard G&#246;ttingen Minipigs A/S, Denmark</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Euthanimal</td>
			<td style="width:191px;"></td>
			<td style="width:149px;"></td>
			<td>pentobarbital</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Ketamine</td>
			<td style="width:191px;">Pfizer</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Midazolam&#160;</td>
			<td style="width:191px;">Hameln Pharmaceuticals</td>
			<td style="width:149px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

exposure of the pig cns for histological analysis: a manual for decapitation, skull opening, and brain removal

Domuzlar insan olmayan primatlar için ekonomik ve etik açıdan uygun yerine büyük hayvan translasyonel sinirbilim araştırmalarında giderek daha popüler hale gelmiştir. domuzun büyük beyin boyutu geleneksel klinik beyin kameralar ve insan klinikten doğrudan kullanımı ve beyin cerrahisi prosedürleri ve ekipmanın test kullanımına izin verir. Daha fazla makroskopik ve histolojik analizi, ancak, domuz, merkezi sinir sistemi (CNS) ve daha sonra beyin çıkarılması postmortem maruz kalmayı gerektirir. Bu merkezi sinir sistemi kalın, kemik kafatası ve omurga ile kapsül olup domuz gibi kolay bir iş değildir. Bu kağıt ve öğretim videonun amacı açığa ve postmortem domuz beyni ve sonraki makroskopik ve histolojik analiz için uygun bozulmamış bir halde hipofiz bezini, nasıl kaldırılacağı tanımlamaktır.

kurumasını doku malzeme önlemek için, sabitleştirici veya makroskopik analizi yapılmıştır hemen sonra izotonik tuzlu su ile dolu bir kavanoz içinde ayrılmıştır beyin ve hipofiz saklanması tavsiye edilir. doku materyali, izotonik tuzlu su depolama oysa yıl süreyle tespit sıvısı içerisinde depolanabilir da bir buzdolabı içinde zamanla doku çürümesine neden olur. Sağlam domuzu beyin doğrudan dondurma <...

Watch this Scientific Journal Video about Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal at JoVE.com

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Bu kağıt ve öğretim videonun amacı ortaya çıkarmak ve daha sonraki makroskopik ve histolojik analiz için uygun bozulmamış bir halde, domuz beyin ve hipofiz bezi otopsi nasıl kaldırılacağı tanımlamaktır.

Histolojik Analiz Domuz MSS Pozlama: Decapitation, Kafatası Açılış İçin El Kitabı ve Beyin Kaldırma

Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to ...

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Anatomy and Physiology

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Unit 1

The Axial Skeleton

SCIENTISTS IN ACTION

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Fare Beyin Uzun vadeli Görüntüleme için Parlak ve Takviyeli inceltilmiş-kafatası Pencere

In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form ...

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

FMRI ve difüzyon ağırlıklı görüntüleme ile Beyin Yapısı ve Fonksiyonu Co-analizi

The study of complex computational systems is facilitated by network maps, such as circuit diagrams. Such mapping is particularly informative when ...

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures

Medial Temporal Lob Yapıların El segmentasyonu için Kapsamlı Protokolü

The present paper describes a comprehensive protocol for manual tracing of the set of brain regions comprising the medial temporal lobe (MTL): amygdala, ...

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Synaptic Modülasyon Analizi<em&gt; Drosophila melanogaster</em&gt; Uzun süreli ışığa maruz kaldıktan sonra Fotoreseptörler

The nervous system has the remarkable ability to adapt and respond to various stimuli. This neural adjustment is largely achieved through plasticity at ...

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

Yüksek üretilen iş analiz Locomotor davranış Drosophila Adası tahlil

Advances in next-generation sequencing technologies contribute to the identification of (candidate) disease genes for movement disorders and other ...

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Sinaptik plastisite akut Hipokampal dilimleri bir küçük hacimli geri dönüşüm-, perfüzyon- ve batma-tipi oda sistemi içinde tutulan kayıt

Even though experiments on brain slices have been in use since 1951, problems remain that reduce the probability of achieving a stable and successful ...

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Roman pasif optik şeffaflık bütün sinir dokusunda hızlı üretim için yöntemleri Temizleme

Since the development of CLARITY, a bioelectrochemical clearing technique that allows for three-dimensional phenotype mapping within transparent tissues, ...

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

Kronik yaygın Marmoset bütün kortikal Electrocorticographic dizide implantasyonu

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

Beyin Yapılarını Hedeflemek ve Kemogenetik Nöromodülasyonu Değerlendirmek için Odaklanmış Ultrason Kaynaklı Kan-Beyin Bariyeri Açılması

Acoustically Targeted Chemogenetics (ATAC) allows for the noninvasive control of specific neural circuits. ATAC achieves such control through a ...

Extraction and Dissection of the Domesticated Pig Brain

Evcilleştirilmiş Domuz Beyninin Ekstraksiyonu ve Diseksiyonu

Use of the pig as a preclinical and translatable animal model has been well-documented and accepted by research fields investigating cardiovascular ...