Results: Both Enrichment Methods Yield Sufficient DNA Highly Enriched for Organellar Sequences and Suitable for NGS
1:51
Organellar DNA Enrichment by Differential Centrifugation (DC)
7:31
Organellar DNA Enrichment by Methyl-fractionation (MF)
Transcript
The overall goal of this experiment is to compare two methods used to isolate high quality organellar DNA, both plastid and mitochondria DNA from plant leaf tissue that is suitable for next-generation sequencing. These methods can help answer key
Sign in or start your free trial to access this content
Sammenligningen og optimeringen af to planteorganelle DNA-berigelsesmetoder er præsenteret: traditionel differentiel centrifugering og fraktionering af det totale gDNA baseret på methyleringsstatus. Vi vurderer den resulterende DNA-mængde og kvalitet, demonstrerer ydeevne ved kortlæsning af næste generations sekventering og diskuterer potentialet til brug i langlæsende enkeltmolekylære sekventering.