Results: Both Enrichment Methods Yield Sufficient DNA Highly Enriched for Organellar Sequences and Suitable for NGS
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Organellar DNA Enrichment by Differential Centrifugation (DC)
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Organellar DNA Enrichment by Methyl-fractionation (MF)
Transcript
The overall goal of this experiment is to compare two methods used to isolate high quality organellar DNA, both plastid and mitochondria DNA from plant leaf tissue that is suitable for next-generation sequencing. These methods can help answer key
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Sammenligningen og optimaliseringen av to organellar DNA-anrikningsmetoder presenteres: Tradisjonell differensial sentrifugering og fraksjonering av totalt gDNA basert på metyleringsstatus. Vi vurderer den resulterende DNA-kvantiteten og kvaliteten, demonstrerer ytelse i kortlestende neste generasjons sekvensering, og drøfter potensialet for bruk i langlestende enkeltmolekylesekvensering.