Preparing Medium and Artificial Sputum for Capillary Tube Loading
2:29
Filling the WinCF Capillary Tubes with Inoculated Artificial Sputum Medium
4:12
Incubation and Imaging the Extraction
5:38
Results: Microbiological Growth in the WinCF Capillary Tubes
6:22
Conclusion
Transcript
The overall goal of this procedure is to mimic the lung environment and grow pathogens in a manner more similar to that from which they cause disease. This method can help answer key questions in the airway microbiology field Such as, how diverse
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The mucus plugged airways of cystic fibrosis (CF) patients are an ideal environment for microbial pathogens to thrive. The manuscript describes a novel method for studying the CF lung microbiome in an environment that mimics where they cause disease and how alterations of chemical conditions can drive microbial dynamics.