Expression of Recombinant HA-MOR1, Cell Fractionation, and Membrane Collection
9:01
Conclusion
7:57
Results: Characterization of G-protein-coupled Receptors by Monitoring Radio-labled GTP Binding
3:55
[35S]GTPγS Binding
6:05
Membrane Filtration
6:59
Liquid Scintillation Counting
Transcript
The overall goal of this procedure is to isolate cellular membranes that contain G-protein-coupled receptors or GPCRs and to study GPCR pharmacology using a functional GTP binding essay. This method can help answer key questions in the field of bi
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Guanosintriphosphat (GTP) -binding er en af de tidligste begivenheder i G-protein-koblet receptor (GPCR) -aktivering. Denne protokol beskriver, hvordan man farmakologisk karakteriserer specifikke GPCR-ligandinteraktioner ved at overvåge bindingen af den radiomærkede GTP-analog, [35S] guanosin-5'-O- (3-thio) triphosphat ([35S] GTPγS), i Svar på en ligand af interesse.