Isolation of RMCE-compatible Knockout Mouse ES Cells
5:22
Isolate RMCE-compatible Knockout ES Cells
7:29
Generation of an RMCE-compatible Targeting Vector Using Recombination Cloning
9:25
RMCE-mediated ES Cell Targeting of Rescue Constructs to the R26 Locus and Differentiation of ES Cells in Embryoid Bodies
12:41
Results: Cystic EB Formation Using Recombinase Mediated Cassette Exchange
14:27
Conclusion
Transcript
The overall goal of this structure-function method is to dissect at the molecular level the role of different protein domains, or disease-relevance mutations, in naive or differentiated mouse embryonic stem cells. This method can help to reveal ho
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Las proteínas a menudo contienen varios dominios que pueden ejercer diferentes funciones celulares. Genes knock-out (KO) no tienen en cuenta esta diversidad funcional. Aquí, se presenta un enfoque basado en la estructura-función de intercambio de casete de recombinación mediada (RMCE) en células madre embrionarias de KO que permite la disección molecular de varios dominios funcionales o variantes de una proteína.