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DOI :
10.3791/55598-v
May 27th, 2017
Chapters
0:05
Title
8:27
Conclusion
7:44
Results: Two-color VCF with Anap and TMR
1:03
Oocyte Preparation and Injection
4:16
VCF Setup
5:35
VCF Recording
この記事では、マレイミド色素の代わりに蛍光非天然アミノ酸(fUAA)を用いて、イオンチャネルの構造的再配列を調べる従来の電圧クランプ蛍光光度法(VCF)の強化について説明します。この手順には、 アフリカツメガエル卵母細胞DNA注入、RNA / fUAA共注入および同時電流および蛍光測定が含まれる。
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