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DOI :
10.3791/55621-v
April 22nd, 2017
Chapters
0:05
Title
8:22
Conclusion
7:12
Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers
0:51
Embryoid Body Formation
1:58
2D Embryoid Body Culture and Detection of Secreted Proteins
3:04
3D Embryoid Body Culture
4:06
Embryoid Body Dissociation
5:56
Analysis of Embryoid Body Formation by Immunofluorescence
我们描述了使用小鼠胚胎干细胞生成二维或三维的胚状体的技术。然后,我们解释如何以诱导胚状体细胞视黄酸,以及如何的神经分化由祖细胞标志物的免疫荧光和免疫印迹来分析其分化状态。
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