Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers
0:51
Embryoid Body Formation
1:58
2D Embryoid Body Culture and Detection of Secreted Proteins
3:04
3D Embryoid Body Culture
4:06
Embryoid Body Dissociation
5:56
Analysis of Embryoid Body Formation by Immunofluorescence
Transcript
The overall goal of this procedure is to analyze the expression and production of genes and protein markers, respectively, of neural differentiation of mouse embryonic stem cells. This method can help determine the mechanism responsible for the in
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Se describe una técnica para el uso de células madre embrionarias murinas para generar dos o tres cuerpos embrioides dimensionales. A continuación, explicamos cómo inducir la diferenciación neural de las células del cuerpo embrioides por ácido retinoico, y cómo analizar su estado de diferenciación de células progenitoras por inmunofluorescencia marcador e inmunotransferencia.