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DOI :
10.3791/55623-v
May 13th, 2017
Chapters
0:05
Title
10:54
Conclusion
9:28
Results: Inter-dye Distance Determination in Single Molecules
1:07
Preparation of Materials
2:57
Pulsed Interleaved Excitation-Multiparameter Fluorescence Detection (PIE-MFD) Instrument Setup
6:14
Burst Integration Fluorescence Lifetime Analysis of Standards
7:34
dsDNA and Protein Sample Measurements and Analysis
本文介绍了单分子水平高精度FRET实验方案。此外,该方法可用于鉴定N-甲基-D-天冬氨酸(NMDA)受体的配体结合结构域中的三种构象状态。确定精确的距离是建立基于FRET实验的结构模型的第一步。
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