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DOI :
10.3791/55655-v
May 18th, 2017
Chapters
0:05
Title
8:50
Conclusion
7:15
Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
0:49
Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
3:16
Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
在这种方法中,我们提出了从多个小鼠组织快速高效地分离中间丝(IF)蛋白质的生物化学方法。分离的IF可用于通过质谱法和其他生化测定法研究翻译后修饰的变化。
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