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DOI :
10.3791/55655-v
May 18th, 2017
Chapters
0:05
Title
8:50
Conclusion
7:15
Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
0:49
Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
3:16
Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
この方法では、複数のマウス組織から中間体フィラメント(IF)タンパク質を迅速かつ効率的に単離するための生化学的手順を提示する。単離されたIFは、質量分析および他の生化学アッセイによる翻訳後修飾の変化を研究するために使用することができる。
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