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DOI :
10.3791/55703-v
June 9th, 2017
Chapters
0:05
Title
1:02
Treatment of Cells and Induction of DNA Damage
12:03
Conclusion
10:34
Results: The PLA Assay Detects Direct Protein-protein Interactions in Suspension Cell Cultures
3:21
Cytocentrifugation, Fixation, and Permeabilization
5:35
Blocking and Primary Antibody Incubation
7:19
PLA Probes, Ligation, and Amplification
9:04
Mounting and Imaging
여기에서는 유전 독성 스트레스에 노출 된 현탁 세포 배양 물에서 ATM과 p53 사이의 직접적인 단백질 - 단백질 상호 작용을 검출하고 시각화하기 위해 현장에서의 근접 연결 분석 (PLA)이 어떻게 사용될 수 있는지 보여줍니다.
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