Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
1:05
Site-directed Mutagenesis of pGEX-Nrf2
3:45
In Vitro Competitive 5' Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
6:13
In Vitro AMPK Activity Assay with a Site-specific Mutant
Transcript
The overall goal of this procedure is to simultaneously assess multiple potential phosphorylation sites and to identify sites for subsequent validation by phosphorylation site mutants. This method can help answer key questions in the biochemistry
Sign in or start your free trial to access this content
Peptidkonkurrenceanalyser anvendes i vid udstrækning i en række molekylære og immunologiske eksperimenter. Dette papir beskriver en detaljeret fremgangsmåde til et in vitro oligopeptidkompeterende kinaseassay og de tilhørende valideringsprocedurer, som kan være nyttige til at finde specifikke phosphoryleringssteder.