Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
1:05
Site-directed Mutagenesis of pGEX-Nrf2
3:45
In Vitro Competitive 5' Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
6:13
In Vitro AMPK Activity Assay with a Site-specific Mutant
Transcript
The overall goal of this procedure is to simultaneously assess multiple potential phosphorylation sites and to identify sites for subsequent validation by phosphorylation site mutants. This method can help answer key questions in the biochemistry
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I test di concorrenza del peptide sono ampiamente usati in una varietà di esperimenti molecolari ed immunologici. Questo documento descrive un metodo dettagliato per un test in vitro di oligopeptide-concorrenza e le relative procedure di convalida, che possono essere utili per trovare siti specifici di fosforilazione.