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JoVE Journal

Biochemistry

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Oligopeptidkompetitionsanalys för fosforyleringsställningsbestämning

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09:16 min

May 18th, 2017

May 18th, 2017

8,312 Views

0:05

Title

8:31

Conclusion

7:39

Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein

1:05

Site-directed Mutagenesis of pGEX-Nrf2

3:45

In Vitro Competitive 5' Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay

6:13

In Vitro AMPK Activity Assay with a Site-specific Mutant

Transcript

The overall goal of this procedure is to simultaneously assess multiple potential phosphorylation sites and to identify sites for subsequent validation by phosphorylation site mutants. This method can help answer key questions in the biochemistry

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Peptidkonkurrensanalyser används ofta i ett flertal molekylära och immunologiska experiment. Detta papper beskriver en detaljerad metod för en in vitro oligopeptidkompetent kinasassay och de associerade valideringsförfarandena, som kan vara användbara för att hitta specifika fosforyleringsställen.

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