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Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

10.6K Views

12:09 min

April 20th, 2017

April 20th, 2017

10,551 Views

0:05

Title

1:03

Insect Cell Culture Maintenance

4:37

Insect Cell Transfection

7:20

Confocal Fluorescence Microscopy

9:33

Results: Protein Localization is Visualized within Live Insect Cells

11:37

Conclusion

Transcript

The overall goal of this procedure is to express fluorescently tagged proteins of interest within commercially available insect cells for the enhanced elucidation of protein function and or cellular trafficking of proteins. This method can help an

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Nonlytic insect cell expression systems are underutilized for production, cellular trafficking/localization, and recombinant protein functional analysis. Here, we describe methods to generate expression vectors and subsequent transient protein expression in commercially available lepidopteran cell lines. The co-localization of Bemisia tabaci aquaporins with subcellular fluorescent marker proteins is also presented.

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