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DOI :
10.3791/55772-v
May 30th, 2017
Chapters
0:05
Title
10:21
Conclusion
8:26
Results: Interaction of Gap Junction, Adhered Junction, and Tight Junction Components in the Murine Mammary Gland
0:48
Identifying Proetin Co-localization
3:41
Studying Protein-protein Interactions (PPIs): Indirect Immunoprecipitation
6:29
Downstream Application: Gel Electrophoresis Followed by Western Blot
细胞间连接是乳腺阶段特异性功能和发育的必需条件。该手稿提供了一个详细的方法,用于研究蛋白质 - 蛋白质相互作用(PPI)和使用鼠乳腺的共定位。这些技术允许调查不同发育阶段的细胞间连接之间的物理联系的动力学。
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