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DOI :
10.3791/55792-v
September 20th, 2017
Chapters
0:05
Title
0:45
Fixation of Staged Embryos
1:52
Sectioning for Immunolabeling
4:04
Confocal Imaging
5:17
Quantifying Microtubules
7:10
De Novo MT Assembly Assay
9:05
Depolymerize Existing MTs
10:26
Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
12:00
Conclusion
ここでは、他の組織に広く適用される、ゼブラフィッシュ脳の発達における微小管の異なる集団を分析するメソッドについて反応について説明します。最初のプロトコルでは、反応の安定性と動的微小管の最適化手法について説明します。第二議定書は、画像し、初期微小管を具体的に数値化する方法を提供します。
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