Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
10:16
Conclusion
8:52
Results: Effects of Chemicls on the Cellular Biological Clock
3:06
Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
5:28
Transient Transfection of Cells with the Circadian Vector
7:46
Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
Transcript
The overall goal of this in vitro bioluminescence assay is to determine the effects of different environmental circadian disruptors and enhancers on the cellular circadian rhythm of mammary epithelial cells from mammary glands at normal or disease
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En in vitro- bioluminescens analyse til at bestemme cellulære døgnrytme i Brystkirtlerne epitelceller er præsenteret. Denne metode udnytter pattedyr celle reporter plasmider udtrykker destabiliseret luciferase under kontrol af periode 2 gen promotor. Det kan tilpasses til andre celletyper til at evaluere orgel-specifikke virkninger på døgnrytme.