Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
10:16
Conclusion
8:52
Results: Effects of Chemicls on the Cellular Biological Clock
3:06
Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
5:28
Transient Transfection of Cells with the Circadian Vector
7:46
Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
Transcript
The overall goal of this in vitro bioluminescence assay is to determine the effects of different environmental circadian disruptors and enhancers on the cellular circadian rhythm of mammary epithelial cells from mammary glands at normal or disease
Sign in or start your free trial to access this content
Bioluminescens i vitro analysen å bestemme mobilnettet døgnrytmen i mammary epitelceller presenteres. Denne metoden bruker pattedyr celle reporter plasmider uttrykke ustabil luciferase under kontroll av periode 2 gen arrangøren. Det kan tilpasses andre celletyper evaluere organ-spesifikke effekter på døgnrytmen.