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In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

8.4K Views

10:36 min

June 20th, 2017

June 20th, 2017

8,366 Views

0:05

Title

1:14

Culturing hCMEC/D3 Immortalized Endothelial Cells

2:08

Preparation of Mouse Brain Endothelial Cells (BECs)

4:35

Single BEC Culture Assay

6:24

Triple Culture Assay

8:13

Quantification

8:44

Results: Effects of oAβ and EGF in Single- and Triple-culture Meshwork Disruption Assays

9:51

Conclusion

Transcript

The goals of this assay are to dissect the role of disease-relevant processes in blood brain-barrier coverage disruption, identify the underlying mechanisms, and test potential treatments in vitro.This method can answer key basic and applied resea

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Maintaining blood-brain barrier coverage is key for the homeostasis of the central nervous system. This protocol describes in vitro techniques to delineate the fundamental and pathological processes that modulate blood-brain barrier coverage.

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