Sign In

JoVE Journal

Developmental Biology

Author Produced

A subscription to JoVE is required to view this content.

live

Speed

×

MEDIA_ELEMENT_ERROR: Format error

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

7.9K Views

07:11 min

December 30th, 2017

December 30th, 2017

7,922 Views

0:00

Title

0:30

Dissection Overview

2:23

Dissection of pupal wings

4:38

Multiple pupae dissection

5:32

Antibody stained pupal wings and PCR

6:23

Conclusions

Transcript

The overall goal of this procedure is a rapid and efficient method to dissect pupal wings of Drosophila suitable for immunodetections or PCR assays. The procedure described here was developed by the demand to find a fast and efficient method to ob

Sign in or start your free trial to access this content

The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More